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HPLC-ECD Analytical Applications

Dopamine (DA) and Serotonin (5-HT) in Microdialysis Samples <PP-ODS Column>

Detection limit 160 amol (0.16 fmol, 30 fg) for both DA and 5-HT
Robust 5-minute analytical method
Clear separation of peaks >Dopamine, Serotonin Chromatogram
Dopamine and Serotonin, Application Note
Related; Find the best catecholamine analysis for your samples (another window will open)>suggestion

Dopamine, Serotonin and Norepinephrine (NE) in Microdialysis Samples <CAX Column>

Simultaneous analysis in 15 minutes
260 amol (50 fg) detection limit for DA and 580 amol (0.1 pg) for NE and 5-HT
Allows for higher sample temporal resolution (15 min) >chromatogram
Simultaneous NE, DA and 5-HT, Application Note
Related; Find the best catecholamine analysis for your samples (another window will open)>suggestion

Acetylcholine (ACh) in Microdialysis Samples <AC-GEL Column>

No cholinesterase inhibitor required for the medial prefrontal cortex.
New improved enzymatic column provides increased sensitivity
Reproducible sensivitiy level of 10 fmol >chromatogram
18 minute run time with internal standard, 13 min without internal standard.
Also allows for the detection of Choline (Ch)
Acetylcholine, Application Note

Glutamate (Glu) in Various Samples <GU-GEL Column>

Flexible analytical method with adaptability to microdialysate, blood and tissue homogenate
Microdialysis sample run time of 10 minutes
Robust detection limit (< 3 fmol). (Microdialysis samples have higher concentrations)> chromatogram
Glutamate Online Enzymatic Assay, Application Note

GABA in Microdialysis Samples <SC-5ODS Column>

Sensitivity level 30 fmol (1 nM x 30 µl).
High specificity. Ca²⁺ free perfusate decreases the peak height.
28 minute method with the incorporation of a switching valve >chromatogram
GABA in Microdialysis Samples, Application Note

Thiols in Plasma, Tissue Samples, Culture Medium and Others <SC-3ODS Column>

New - ECD with a gold working electrode
Highly sensitive with 1 fmol detection limit for GSH and 0.5 fmol for -SH compounds >chromatogram
Thiols Detection, Application Note

3-Nitrotyrosine in Hydrolyzed Protein <Model PEC-510, SC-5ODS Column>

Methodology employs dual ECD's with preelectrolysis cell.
16-minute method with a 0.5 fmol (10 pM x 50 µl) sensitivity level
Additional unit, PEC-500 pre-electrolysis system to the HTEC-500 is required. >chromatogram

Monoamine and Metabolite Analysis from Tissue Homogenates <SC-3ODS Column>

Comprehensive analytical methodology
Easy sample preparation >chromatogram
Related; Find best catecholeamine analysis for your samples (another window will open)>suggestion

Catecholamines in Plasma <CA-5ODS Column>

Approx. 0.5 fmol (=0.1 pg) sensitivity. 20 min for one sample.>chromatogram
Require sample preparation with activated alumina

Catecholamine Analysis in Plasma or Urine, Application Note

Related; Find best catecholeamine analysis for your samples (another window will open)>suggestion

Cannot find what your are looking for?

Eicom has more analytical applications. Simply ask us! >contact us

Analytical Applications are Part of Our Product Line

In house development

All applications listed here were developed or tested repeatedly at Eicom's lab. We provide technical support for all of our analytical applications.

No problem starting new applications

Thanks to the elimination of the pulse damper, the minimum dead volume, and quickly stabilizing detector of Eicom's HTEC-500, switching applications is significantly faster and easier.

Eicom model HTEC-500, HPLC-ECD system works for neurotransmitter assays, phenotype studies, food analysis, and environmental assessments. Our major analytical applications are listed here. For all applications, more detailed Application Manuals are available. If you are an Eicom user, you can download them from User Support.

Related Pages

-HTEC-500 Features

-HTEC-500 Details

-HTEC-500 Specifications

-Analytical Applications

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-Support Philosophy

-ECD Fundamentals

-Microdialysis Products