HPLC-ECD Analytical Applications

Acetylcholine (ACh) in Microdialysis Samples

• No cholinesterase inhibitor required for the medial prefrontal cortex. 
• New improved enzymatic column provides increased sensitivity
• Reproducible sensivitiy level of 5 fmol >chromatogram
• 18 minute run time with internal standard, 13 min without internal standard.
• Also allows for the detection of Choline 
•  Acetylcholine, Application Note

Coenzyme Q10 and Vitamin K1 (Quinones) with Preelectrolysis Cell 

• No platinum black is required
• Higher reproducibility
• Lower running cost 
•  CoQ10 and VK1 Application Note 

Dopamine (DA) and Serotonin (5-HT) in Microdialysis Samples

• Detection limit 160 amol (0.16 fmol, 30 fg) for both DA and 5-HT
• Robust 5-minute analytical method
• Clear separation of peaks >Dopamine, Serotonin Chromatogram
•  Dopamine and Serotonin, Application Note 
• Related; Find the best catecholamine analysis for your samples (another window will open)>suggestion 

Dopamine, Serotonin and Norepinephrine (NE) in Microdialysis Samples

• Simultaneous analysis in 15 minutes
• 260 amol (50 fg) detection limit for DA and 580 amol (0.1 pg) for NE and 5-HT
• Allows for higher sample temporal resolution (15 min) >chromatogram
•  Simultaneous NE, DA and 5-HT, Application Note 
• Related; Find the best catecholamine analysis for your samples (another window will open)>suggestion 

Glutamate (Glu) and GABA in 12 min, Various Samples

• Flexible analytical method with adaptability to microdialysate and tissue homogenate
  
• No UHPLC or two systems required.
•  Glu GABA 12 min, Application Note 
   

Glutamate (Glu) in Various Samples

• No precolumn derivatization required
• Flexible analytical method with adaptability to microdialysate, blood and tissue homogenate
• Microdialysis sample run time of 10 minutes
• Robust detection limit (< 3 fmol). (Microdialysis samples have higher concentrations)> chromatogram
•  Glutamate Online Enzymatic Assay, Application Note  

GABA in Microdialysis Samples

• Sensitivity level 30 fmol (1 nM x 30 µl).
• High specificity. Ca²⁺ free perfusate decreases the peak height.
• 28 minute method with the incorporation of a switching valve >chromatogram
•  GABA in Microdialysis Samples, Application Note  

Thiols in Plasma, Tissue Samples, Culture Medium and Others

• New - ECD with a gold working electrode
• Highly sensitive with 1 fmol detection limit for GSH and 0.5 fmol for -SH compounds >chromatogram
•  Thiols Detection, Application Note  

3-Nitrotyrosine in Hydrolyzed Protein

• Methodology employs dual ECD's with preelectrolysis cell.
• 16-minute method with a 0.5 fmol (10 pM x 50 µl) sensitivity level
• Additional unit, PEC-500 pre-electrolysis system to the HTEC-500 is required. >chromatogram 

Monoamine and Metabolite Analysis from Tissue Homogenates

• Comprehensive analytical methodology
• Easy sample preparation >chromatogram
• Related; Find best catecholamine analysis for your samples (another window will open)>suggestion 

Catecholamines in Plasma

• Approx. 0.5 fmol (=0.1 pg) sensitivity. 20 min for one sample.>chromatogram
• Require sample preparation with activated alumina
•  Catecholamine Analysis in Plasma or Urine, Application Note
• Related; Find best catecholamine analysis for your samples (another window will open)>suggestion

Cannot find what your are looking for?

Eicom has more analytical applications. Simply ask us! >contact us

Analytical Applications are Part of Our Product Line

In house development All applications listed here were developed or tested repeatedly at Eicom's lab. We provide technical support for all of our analytical applications.

No problem starting new applications Thanks to the elimination of the pulse damper, the minimum dead volume, and quickly stabilizing detector of Eicom's HTEC-500, switching applications is significantly faster and easier. For users, more detailed Application Manuals are available.

HPLC-ECD HTEC-500 

HPLC-ECD 700 Series

If you experience difficulty opening PDF files, please download the latest version of Acrobat Reader.