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High sensitivity

HPLC-ECD System

HTEC-600

​“ Ultra-High Sensitivity and Stability 
★ Ultrasensitive, 30 fg of dopamine, serotonin
★ Eicomʼ s Innovative Cell Design System Design with Pulsation Free Pump
★ Temperature Regulated System with Both Cooler and Heater Optimized Applications for the ECD

 
As you can see, the sensitivity could not be achieved only by the detector.

All-In-One With A Small Footprint
The liquid feed pump, degasser, thermostatic chamber, and electrochemical detector
have been integrated. This makes it possible to make use of analysis applications for other detectors, such as flow speeds exceeding 500 μL/min.
* The new model can be used with long columns which could not be placed in a chamber for the previous model, allowing it to support a wider range of analysis applications.

HTEC-600-12.png

​“ PURGE function

PURGE function 
This function allows for high-speed liquid feeding. The operation time can be set, and there is no need to be concerned over liquid drying out.
Air bleeding and liquid exchanging can be performed automatically, significantly improving pump errors (the most frequent general issue with HPLC).

★ TIMER function
This function can turn the pump and ECD ON/OFF individually using time-based control. The operation time can be controlled,
allowing consumption during the mobile phase to be minimized. This function can be used to operate the pump and ECD efficiently,
allowing for trouble-free sample measurement.

★ L.SWITCH function

ELS-500 can be controlled from HTEC-600. Eliminating contamination during the later stage of a chromatogram can streamline amino acid
analysis or analysis for samples in which contamination is produced comparatively more often. The ease-of-use provided by ELS-500 is a
major advantage when conducting experiments.

Easy to Maintain
 
The simple compact design of the HTEC-600 (HPLC-ECD) allows for easy maintenance. No tools to access the working electrode/ Easy
to clean or change the working electrode.

★Dopamine ★DOPAC ★HVA ★3MT ★L-DOPA ★Norepinephrine ★MHPG ★Normetanephrine ★Epinephrine 
★Serotonin ★5-HIAA ★Melatonin ★Phenols ★acetylcholine ★Colin ★Hydroxyl radical ★Ascorbic acid ★Tocopherol 
★Estradiol ★80H-dG ★Nitro tyrosine ★GSH ★GSSG ★Aspartic acid  ★Glutamic acid ★glutamine ★glycine  ★Tannins 
★Alanine ★GABA etc


There is another. For more details contact us!

“Specification


Detector★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★

Electrochemical Cell      Amperometric
Applied Voltage Range         0 ~ ±2,000 mV、1 mV step
Working electrode method        Thin layer type
Working Electrode                    Graphite (standard)、Pure graphite、Grassy carbon
                                                Platinum、Gold、Silver
Working electrode gasket          Made by TFE thickness of 25 μm or 50 μm
Reference Electrode                 Silver/Silver chloride
Counter Electrode                     SUS316
Wetted part(material)                PEEK、SUS316
Recorder Output                       ±10 V analog (0.1 nA = 1 mV)
Time constant                          1.0 sec、1.5 sec、3.0 sec
Output signal                            SIGNAL IN:Contact signal 300 msec over detect
                                                M.INJECTOR SIGNAL IN:open/ close detected in 300 sec after signal switching


Pump★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★

1 stroke capacity                      80 μL
Piston material                          Sapphire
Set flow rate range                    1 ~ 3000 μL/min
Stable flow range                      100 ~ 2000 μL/min
Wetted part(material)                PEEK、Sapphire、Ruby、PTFE、PCTFE
Withstand voltage                     20 MPa


Degasser★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★

Number of passes                     2 channels
Internal capacity                       Upper row:about 300 μL/ flow channel
                                                 Lower row:about 7.5 mL/ flow channel


Constant temperature oven★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★

Temperature Range                   15 ~ 50℃ (1℃step)
Temperature Accuracy               ±0.1℃


Size, weight, power★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★★

Size (W x D x H)                        290 W x 380 D x 435 H mm
Weight                       23.2 kg
Power AC                                  100 ~ 240 V 50/60 Hz 300 VA

2022
・Glucose infusion suppresses acute restraint stress-induced peripheral and central sympathetic responses in rats
・Investigating the Influence of GABA Neurons on Dopamine Neurons in the Ventral Tegmental Area Using Optogenetic Techniques

・The efficacy of duloxetine depends on spinal cholinergic plasticity in neuropathic pain model rats
・Behavioral and neurochemical effects of novel N-Benzyl-2-phenylethylamine derivatives in adult zebrafish
2021
・Spike firing attenuation of serotonin neurons in learned helplessness rats is reversed by ketamine

・l-Menthol increases extracellular dopamine and c-Fos-like immunoreactivity in the dorsal striatum, and promotes ambulatory activity in mice
・P2X2 receptors supply extracellular choline as a substrate for acetylcholine synthesis
・Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice
・The essential role of transcription factor Pitx3 in preventing mesodiencephalic dopaminergic neurodegeneration and maintaining neuronal
  subtype identities during aging

・Ninjin'yoeito, a traditional Japanese medicine, increases dopamine content in PC12 cells
・Preventative Effects of 1-Methyl-1,2,3,4- Tetrahydroisoquinoline Derivatives (N-Functional Group Loading) On MPTP-Induced Parkinsonism
  In Mice

・Simultaneous serotonin and dopamine monitoring across timescales by rapid pulse voltammetry with partial least squares regression
・A novel in vitro assay model developed to measure both extracellular and intracellular acetylcholine levels for screening cholinergic
 agents 
Hormonal and Neurological Aspects of Dog Walking for Dog Owners and Pet Dogs
・Ninjin’yoeito, a traditional Japanese medicine, increases dopamine content in PC12 cells
・FFAR1/GPR40 Contributes to the Regulation of Striatal Monoamine Releases and Facilitation of Cocaine-Induced Locomotor Activity
 in Mice

・α-Synuclein-induced dysregulation of neuronal activity contributes to murine dopamine neuron vulnerability
・Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide
  in the prolonged chronic unpredictable stress model

・Genetic dissection identifies Necdin as a driver gene in a mouse model of paternal 15q duplications
・Schizophrenia-Like Behavioral Impairments in Mice with Suppressed Expression of Piccolo in the Medial Prefrontal Cortex
・Vitamin B6 deficiency hyperactivates the noradrenergic system, leading to social deficits and cognitive impairment
・In Vivo Monitoring of Acetylcholine Release from Nerve Endings in Salivary Gland
・The Effects of Acute Glucoprivation on Adrenomedullary Function in SHR and WKY Rats
・Mice with mutations in Trpm1, a gene in the locus of 15q13.3 microdeletion syndrome, display pronounced hyperactivity and decreased  anxiety-like behavior
・Activation of the cardiac non-neuronal cholinergic system prevents the development of diabetes-associated cardiovascular complications
・Dual actions of 5-MeO-DIPT at the serotonin transporter and serotonin 5-HT1A receptor in the mouse striatum and prefrontal cortex
・Decreased content of ascorbic acid (vitamin C) in the brain of knockout mouse models of Na+,K+-ATPase-related neurologic disorders
・Essential role for autophagy protein VMP1 in maintaining neuronal homeostasis and preventing axonal degeneration
・Non-neuronal cardiac acetylcholine system playing indispensable roles in cardiac homeostasis confers resiliency to the heart
・Beta-blockade Prevents Coronary Macro and Microvascular Dysfunction Induced by a High Salt Diet and Insulin Resistance in the
 Goto-Kakizaki Rat

・Selective inhibition of monoacylglycerol lipase is associated with passive coping behavior and attenuation of stress-induced dopamine
  release in the medial prefrontal cortex

2020
・Mice with Mutations in Trpm1, a Gene within the locus of 15q13.3 Microdeletion Syndrome, Display Pronounced Hyperactivity and
 Decreased AnxietyLike Behavior.

・Understanding complex dynamics of behavioral, neurochemical and transcriptomic changes induced by prolonged chronic
 unpredictable stress in zebrafish

・Mice carrying a schizophrenia-associated mutation of the Arhgap10 gene are vulnerable to the effects of methamphetamine treatment
  on cognitive function: association with morphological abnormalities in striatal neurons

・Noradrenaline enhances the excitatory effects of dopamine on medial prefrontal cortex pyramidal neurons in rats
・Absence of methamphetamine‐induced conditioned place preference in weaver mutant mice
・Monoamine and genome-wide DNA methylation investigation in behavioral addiction
・Highly Efficient Conversion of Motor Neuron-Like NSC-34 Cells into Functional Motor Neurons by Prostaglandin E2
・Monoamine and genome-wide DNA methylation investigation in behavioral addiction
・Gamma oryzanol impairs alcohol-induced anxiety-like behavior in mice via upregulation of central monoamines associated with Bdnf
 and Il-1β signaling

・Participation of the nucleus accumbens dopaminergic system in the antidepressant-like actions of a diet rich in omega-3
 polyunsaturated fatty acids

・Early weaning augments the spontaneous release of dopamine in the amygdala but not the prefrontal cortex: An in vivo microdialysis
 study of male rats

・VGLUT2-expressing neurons in the vestibular nuclear complex mediate gravitational stress-induced hypothermia in mice
・Brain Angiopathy and Impaired Glucose Metabolism in Model Mice with Psychiatric-Related Phenotypes
・Tyr-Trp administration facilitates brain norepinephrine metabolism and ameliorates a short-term memory deficit in a mouse model of
  Alzheimer’s disease

・Twelve-Month Studies on Perilla Oil Intake in Japanese Adults—Possible Supplement for Mental Health​
・The Effect of Stressor Controllability on Social Hedonic Preference in Laboratory Rats
・Gamma Oryzanol Alleviates High-Fat Diet-Induced Anxiety-Like Behaviors Through Downregulation of Dopamine and Inflammation in
  the Amygdala of Mice

・Direct visualization of an antidepressant analog using surface-enhanced Raman scattering in the brain
・Prolyl-hydroxyproline, a collagen-derived dipeptide, enhances hippocampal cell proliferation, which leads to antidepressant-like
 effects in mice

・Protein Deficiency-Induced Behavioral Abnormalities and Neurotransmitter Loss in Aged Mice Are Ameliorated by Essential Amino Acids
・Angiopathy with impaired glucose logistics observed in schizophrenia-like model mice
・Trace Amine-Associated Receptor 5 Provides Olfactory Input Into Limbic Brain Areas and Modulates Emotional Behaviors and Serotonin
  Transmission

・Activation of mGluR5 and NMDA Receptor Pathways in the Rostral Ventrolateral Medulla as a Central Mechanism for Methamphetamine
 -Induced Pressor Effect in Rats

2019
・(R)-Ketamine Induces a Greater Increase in Prefrontal 5-HT Release Than (S)-Ketamine and Ketamine Metabolites via an AMPA
 Receptor-Independent Mechanism

・Kappa Opioid Receptors Drive a Tonic Aversive Component of Chronic Pain
・Parkinson’s disease recovery by GM1 oligosaccharide treatment in the B4galnt1+/− mouse model
・Hyperreflexia induced by XLR-11 smoke is caused by the pyrolytic degradant
2018
・Thiamine tetrahydrofurfuryl disulfide promotes voluntary activity through dopaminergic activation in the medial prefrontal cortex
・Effects of Memantine on Nitric Oxide Production and Hydroxyl Radical Metabolism during Cerebral Ischemia and Reperfusion in Mice
2017
・Overexpression of transmembrane protein 168 in the mouse nucleus accumbens induces anxiety and sensorimotor gating deficit
・Prenatal nicotine exposure decreases the release of dopamine in the medial frontal cortex and induces atomoxetine-responsive
  neurobehavioral deficits in mice

2016
・The Role of Spinal Dopaminergic Transmission in the Analgesic Effect of Nefopam on Rat Inflammatory Pain

・Abuse-Related Neurochemical Effects of Para-Substituted Methcathinone Analogs in Rats: Microdialysis Studies of Nucleus Accumbens
  Dopamine and Serotonin

・Novel psychoactive benzofurans strongly increase extracellular serotonin level in mouse corpus striatum
・Sex Differences in Abuse-Related Neurochemical and Behavioral Effects of 3,4-methylenedioxymethamphetamine (MDMA) in Rats
・Brain regions and monoaminergic neurotransmitters that are involved in mouse ambulatory activity promoted by bupropion
2015
Injection of corticotropin-releasing hormone into the amygdala aggravates visceral nociception and induces noradrenaline release in rats
2014
・Pregerminated Brown Rice Enhanced NMDA Receptor/CaMKIIα Signaling in the Frontal Cortex of Mice 
Monitoring Serotonin: Why Faster is Better
2013
・Physiologically Relevant Changes in Serotonin Resolved by Fast Microdialysis
・Pain-Related Depression of the Mesolimbic Dopamine System in Rats: Expression, Blockade by Analgesics, and Role of Endogenous
 κ-opioids

・Symmetrical Serotonin Release during Asymmetrical Slow-Wave Sleep: Implications for the Neurochemistry of Sleep–Waking States
2011
・Oral administration of glycine increases extracellular serotonin but not dopamine in the prefrontal cortex of rats
2009
・GABAergic transmission in the rat ventral pallidum mediates a saccharin palatability shift in conditioned taste aversion
2007
・Cortical Acetylcholine Release Is Lateralized during Asymmetrical Slow-Wave Sleep in Northern Fur Seals