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ENO-30

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» Suitable for all biological fluids( 1-50 µL injections)

» Both Nitrite and Nitrate from single measurement
» Sensitivity is better than 0.1 pmol (10 nM for 10
» Easily automated to run 144 samples per day

​Nitrite and Nitrate Analyzer

The ENO-30 continues the tradition of Eicom’s successful
ENO-30 NOx analyzer. The ENO-30 system is a specialized
HPLC for separating and detecting nitrite and nitrate in various
biological fluids. The system makes use of a simple Griess
reaction, in which nitrite reacts to form a light absorbing
compound that can easily be detected. However, compared to a typical plate or tube based Griess assay, the ENO-30 greatly enhances
sensitivity and reproducibility. In addition, high throughput analysis is achieved by pairing the instrument with an HPLC autosampler,
an impossible feat for the gas phase chemiluminescent detectors sometimes used to detect nitric oxide. 
Suitable for all biological fluids(1-50 μL injections)

Nitrite and Nitrate Appear as Two Peaks
Each injection measures both nitrite and nitrate.

Peak sizes are proportional to concentration in the sample.

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Perfect for Biological Samples
Sensitive enough for small volume biological samples. The typical sample
is just 1-50 μ L of blood plasma, urine, saliva, breathe condensate, tissue
homogenate, cell culture supernatant, or microdialysate. Simple sample
preparation consist of the addition of an equal volume of methanol to
inactive and precipitate proteins, whereas low protein samples can be
direct injected without prior manipulation.

How does it work?

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​journal article

2022
・Effect of fat reformulated dairy food consumption on postprandial flow-mediated
 dilatation and cardiometabolic risk biomarkers
  compared with conventional dairy:
​ a randomized, controlled trial
2021
・Influence of antidepressants on plasma levels of nitric oxide metabolites in patients with major depressive disorder

・Resuscitative efficacy of hemoglobin vesicles for severe postpartum hemorrhage in pregnant rabbits
・Spontaneous pulmonary emphysema in mice lacking all three nitric oxide synthase isoforms
・Balance of Drug Residence and Diffusion in Lacrimal Fluid Determine Ocular Bioavailability in In Situ Gels Incorporating Tranilast Nanoparticles
・Plasma Nitrate Levels Are Related to Metabolic Syndrome and Are Not Altered by Treatment with DPP-4 Inhibitor Linagliptin:
   A Randomised, Placebo-Controlled Trial in Patients with Early Type 2 Diabetes Mellitus

・Supplemental nitrite increases choroidal neovascularization in mice
・Effects of inorganic nitrate on ischaemia-reperfusion injury after coronary artery bypass  surgery
・The effect of chlorhexidine mouthwash vs propolis mouthwash on the nitrate-reducing activity of oral bacteria and vascular control in
  healthy individuals

・Red Blood Cell and Endothelial eNOS Independently Regulate Circulating Nitric Oxide Metabolites and Blood Pressure

・Direct comparison of inorganic nitrite and nitrate on vascular dysfunction and oxidative damage in experimental arterial hypertension
・Preventive effects of nitrate-rich beetroot juice supplementation on monocrotaline- induced pulmonary hypertension in rats
・Endogenous generation of nitro-fatty acid hybrids having dual nitrate ester (RONO2) and nitroalkene (RNO2) substituents
・Divergent trajectories of cellular bioenergetics, intermediary metabolism and systemic redox status in survivors and non-survivors
  of critical illness

・Oral administration of l-citrulline alters the vascular delivery of substances to rat skeletal muscles
・Temporal relationship between systemic endothelial dysfunction and alterations in erythrocyte function in a murine model of chronic heart failure

・Balance of Drug Residence and Diffusion in Lacrimal Fluid Determine Ocular  Bioavailability in In Situ Gels Incorporating Tranilast
  Nanoparticles

・Plasma Nitrate Levels Are Related to Metabolic Syndrome and Are Not Altered by  Treatment with DPP-4 Inhibitor Linagliptin: A Randomised,
 Placebo-Controlled Trial in  Patients with Early Type 2 Diabetes Mellitus

・Supplemental nitrite increases choroidal neovascularization in mice
Effects of inorganic nitrate on ischaemia-reperfusion injury after coronary artery bypass  surgery
・The effect of chlorhexidine mouthwash vs propolis mouthwash on the nitrate-reducing  activity of oral bacteria and vascular control in
    healthy individuals

・Red Blood Cell and Endothelial eNOS Independently Regulate Circulating Nitric Oxide Metabolites and Blood Pressure
・Direct comparison of inorganic nitrite and nitrate on vascular dysfunction and oxidative  damage in experimental arterial hypertension
・Preventive effects of nitrate-rich beetroot juice supplementation on monocrotaline- induced pulmonary hypertension in rats
・Endogenous generation of nitro-fatty acid hybrids having dual nitrate ester (RONO2) and  nitroalkene (RNO2) substituents
・Divergent trajectories of cellular bioenergetics, intermediary metabolism and systemic redox status in survivors and non-survivors
  of critical illness

・Oral administration of l-citrulline alters the vascular delivery of substances to rat skeletal muscles
・Temporal relationship between systemic endothelial dysfunction and alterations in erythrocyte function in a murine model of chronic heart failure

2020
・Endothelial Cell Cystathionine γ-Lyase Expression Level Modulates Exercise Capacity, Vascular Function, and Myocardial Ischemia
  Reperfusion Injury

・Plant polyphenols Morin and Quercetin rescue nitric oxide production in diabetic mouse aorta through distinct pathways
・A randomized controlled trial of nitrate supplementation in well-trained middle and older-aged adults

・Muscle fiber type differences in nitrate and nitrite storage and nitric oxide signaling in rats
・Dose-response effect of dietary nitrate on muscle contractility and blood pressure in older subjects
・Endothelial Cell Cystathionine γ-Lyase Expression Level Modulates Exercise Capacity, Vascular Function, and Myocardial Ischemia
  Reperfusion Injury

・Plant polyphenols Morin and Quercetin rescue nitric oxide production in diabetic mouse  aorta through distinct pathways
・A randomized controlled trial of nitrate supplementation in well-trained middle and older-aged adults
・Muscle fiber type differences in nitrate and nitrite storage and nitric oxide signaling in  rats

・Dose-response effect of dietary nitrate on muscle contractility and blood pressure in  older subjects
・Anaemia is associated with severe RBC dysfunction and a reduced circulating NO pool: vascular and cardiac eNOS are crucial
  for the adaptation to anaemia

・Unexpected effects of long-term treatment with acetylsalicylic acid on late phase of  pulmonary metastasis in murine model of orthotopic breast cancer

・Reformulation initiative for partial replacement of saturated with unsaturated fats in dairy  foods attenuates the increase in LDL cholesterol and improves flow-mediated dilatation  compared with conventional dairy: the randomized, controlled REplacement of  SaturatEd fat in dairy on Total cholesterol (RESET) study

・Loss of circadian gene Bmal1 in the collecting duct lowers blood pressure in male, but  not female, mice

・The Role of Dietary Nitrate in Regulation of Blood Pressure and Metabolism

・Acute beetroot juice supplementation improves exercise tolerance
 and cycling efficiency in adults with obesity


・Unexpected effects of long-term treatment with acetylsalicylic acid on late phase of  pulmonary metastasis in murine model of orthotopic breast cancer

・Beetroot juice lowers blood pressure and improves endothelial function in pregnant  eNOS-/-mice:importance of nitrate-independent effects


・Reformulation initiative for partial replacement of saturated with unsaturated fats in dairy
  foods attenuates the increase in LDL cholesterol and improves flow-mediated dilatation
  compared with conventional dairy: the randomized, controlled REplacement of
  SaturatEd fat in dairy on Total cholesterol (RESET) study


・Unexpected effects of long-term treatment with acetylsalicylic acid on late phase of
  pulmonary metastasis in murine model of orthotopic breast cancer


・Anaemia is associated with severe RBC dysfunction and a reduced circulating NO pool:
  vascular and cardiac eNOS are crucial for the adaptation to anaemia​ 


2019
BEET-ING MUSCLE DYSFUNCTION AND EXERCISE INTOLERANCE IN PULMONARY HYPERTENSION

Effect of Yokukansan on Nitric Oxide Production and Hydroxyl Radical Metabolism During Cerebral Ischemia and Reperfusion in Mice

Grape Extract from Chardonnay Seeds Restores Deoxycorticosterone Acetate–Salt-Induced Endothelial Dysfunction and Hypertension in Rats

Endothelial GLP-1 (Glucagon-Like Peptide-1) Receptor Mediates Cardiovascular Protection by Liraglutide In Mice With Experimental Arterial Hypertension